The secretion of plasminogen activators by human myeloid leukemic cells in vitro.
نویسندگان
چکیده
Peripheral blood cell preparation from 23 normal subjects and 72 patients with acute and 32 patients with chronic myeloid leukemia were cultured in vitro and released plasminogen activators were analyzed. The quantity of plasminogen activator secreted by leukemic cells varied widely and could not be correlated with the clinical severity of the disease. Immunochemical and electrophoretic techniques have been used to show that normal peripheral blood granulocytes released exclusively urokinase-like plasminogen activator, whereas leukemic cells secreted either urokinase or a tissue activator-like enzyme. The molecular species of enzyme released by acute myeloid leukemic cells may serve as a diagnostic marker of relevance to the management of this disease, since patients with acute myeloid leukemia whose cells released only tissue plasminogen activator did not respond to combination chemotherapy. Tissue plasminogen activators released by leukemic cells may display an unusual electrophoretic pattern that resembles that shown by urokinase. Immunochemical procedures are therefore essential for the correct identification of these enzymes.
منابع مشابه
پتانسیل آنتیپلاسمینوژن منوکلونال آنتیبادی در دستکاری دو سیستم فیبرینولیز و آنژیوژنز
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متن کاملRegulation and secretion of plasminogen activators and their inhibitors in a human leukemic cell line (K562).
The secretion of tissue plasminogen activator (t-PA), urokinase (u-PA) and their inhibitors by the human leukemia cell line K562 was examined. K562 cells normally secrete both t-PA and u-PA in a ratio of 3:1. After addition of 10 or 1 ng/mL phorbol myristate acetate (PMA) to K562 cells, a marked decrease in enzymatic activity is observed in the medium. However, when t-PA antigen rather than act...
متن کاملاثر آنتی بادی های منوکلونال ضد پلاسمینوژن انسانی بر فعال شدن گلو-پلاسمینوژن انسانی بوسیله فعال کننده های پلاسمینوژن
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This investigation was undertaken to examine the extent to which leukemic cell functions are susceptible to regulation in vitro and to investigate their heterogeneity in this regard. Since plasminogen activator release is known to be a modulatable cellular function that can be influenced by antiinflammatory steroids and tetradecanoyl phorbol acetate (TPA), the effect of these two compounds on t...
متن کاملDifferentiation-linked secretion of urokinase and tissue plasminogen activator by normal human hemopoietic cells
Previous studies have shown that the response of patients with acute myeloid leukemia to induction chemotherapy can be predicted by the species of plasminogen activator that their cells secrete. Patients whose cells secreted tissue plasminogen activator (tPA) only failed to respond to combination chemotherapy. Individuals whose leukemic cells display features of the early progenitor phenotype a...
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ورودعنوان ژورنال:
- Blood
دوره 61 3 شماره
صفحات -
تاریخ انتشار 1983